REAGENT or RESOURCE	SOURCE	IDENTIFIER
ExpiCHO Expression System Kit	Thermo Fisher Scientific	Cat# A29133
Expi293 Expression System Kit	ThermoFisher	Cat# A14635
Phosphate-buffered saline (PBS)	Thermo Fisher Scientific	Cat# 14040133
Ni Sepharose excel resin	Cytiva	Cat# 17371202
CaptureSelect CH1-XL Affinity Matrix	Thermo Fisher Scientific	Cat# 1943462010
Chemicals and recombinant proteins
Sodium chloride (NaCl)	Sigma-Aldrich	Cat# S9888
Concentrated hydrochloric acid (HCl)	Sigma-Aldrich	Cat# H1758
Bovine Serum Albumin (BSA)	Sigma-Aldrich	Cat# A9418
Tween 20	Fisher Scientific	Cat# BP337-500
Critical commercial assays
In-Fusion HD Cloning Kit	Takara	Cat# 639647
KOD Hot Start DNA Polymerase	EMD Millipore	Cat# 71086-3
PCR Clean-Up and Gel Extraction Kit	Clontech Laboratories	Cat# 740609.250
QIAprep Spin Miniprep Kit	Qiagen	Cat# 27106
NucleoBond Xtra Maxi	Clontech Laboratories	Cat# 740414.100
Deposited data
Collection of antibody information	This study	Table S1
Custom scripts	This study	https://doi.org/10.5281/zenodo.6370701
Cell lines
ExpiCHO cells	Thermo Fisher Scientific	Cat# A29127; RRID:CVCL_5J31
Expi293F cells	Thermo Fisher Scientific	Cat# A14527; RRID:CVCL_D615
Recombinant DNA
phCMV3-COVOX-253 Fab heavy chain	This study	N/A
phCMV3-COVOX-253 Fab light chain	This study	N/A
phCMV3-SARS-CoV-2-RBD	(Wu et al., 2020b)	N/A
Software and algorithms
Octet analysis software 9.0	Fortebio	N/A
Python	https://www.python.org/	N/A
R	https://www.r-project.org/	N/A
IgBLAST	(Ye et al., 2013)	N/A
Logomaker	(Tareen and Kinney, 2020)	N/A
ANARCI	(Dunbar and Deane, 2016)	N/A
MAFFT	(Katoh and Standley, 2013)	N/A
FastTree	(Price et al., 2010)	N/A
ggtree	(Yu, 2020)	N/A
PyIR	(Soto et al., 2020)	N/A
TensorFlow	(Abadi et al., 2016)	N/A
Other
Fab-CH1 2nd generation (FAB2G) biosensors	ForteBio	Cat# 18-5019