Figure 1.

Creation of beta cell specific PFKM null line. $\beta$-PFKM-KO mice were generated using CRISPR-Cas9 to flox exon 3 of the Pfkm gene (A). Employing this method, we were able to selectively delete the Pfkm mRNA transcript in KO mouse islets, while no statistically significant difference in Pfkp mRNA was observed between wild-type controls and the KO islets (B). Western blot analysis done using a PFKM-specific antibody confirmed the loss of the PFKM protein in the KO islets, while reduced PFKM protein was evident in islets from heterozygous mice (C). To see this figure in color, go online.