Fig. 2.

Effect of the R274W variant on SHARPIN function. a This schematic diagram shows the domain architecture of the SHARPIN protein and the positions of the variants on which our current and previous studies were focused. UBL, ubiquitin-like domain; NZF, Nlp4 zinc finger domain. b NF-κB activity in HEK293 cells under TNF-α–induced activation was determined via luciferase assay, which was performed three times with five replicates in each assay. WT wild-type, GR G186R, RW R274W. *P < 0.05; **P < 0.005. c–e Localization in HEK293 cells of Myc-SHARPIN (c) wild-type, (d) G186R, and (e) R274W was visualized via immunocytochemistry. Scale bar, 10 µm