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. 2022 Mar 24;12:5131. doi: 10.1038/s41598-022-09214-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Relative expression levels (Mean ± SE) of bta-miR-26a, bta-miR-142, bta-miR-146a and bta-miR-223 in milk samples with CMT score 0, 1 or > 1 that were collected from individual mammary quarters of cows at different lactations (1, 2, and 3/4). An effect of lactation number was significant for miR-26, miR-142 and miR-146a (P < 0.005), for which miRNA levels were higher during lactation 1 than during subsequent lactations (P < 0.05). For miR-142 and miR-146a, a CMT x lactation number interaction was also detected (P < 0.01); significant differences within CMT scores that were responsible for such interactions are indicated by different letters (a, b).