Table 2.
Genes | Primer sequence 5ʹ → 3ʹ | |
---|---|---|
Primers used for screening | ||
Reference gene | 16SrRNA |
F: TACATGCAAGTCGAGCGAAC R: AATCATTTGTCCCACCTTCG |
Biofilm genes | icaD |
F: ACCCAACGCTAAAATCATCG R: GCGAAAATGCCCATAGTTTC |
FnbA |
F: AAATTGGGAGCAGCATCAGT R: GCAGCTGAATTCCCATTTTC |
|
EbpS |
F: GGTGCAGCTGGTGCAATGGGTGT R: GCTGCGCCTCCAGCCAAACCT |
|
Vancomycin resistance gene | VanB |
F: GTG ACA AAC CGG AGG CGA GGA R: CCG CCA TCC TCC TGC AAA AAA |