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. 2022 Mar 24;12:5094. doi: 10.1038/s41598-022-08849-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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DNA and total sGAG produced (released and retained) content of MSCs seeded in fibrin polyurethane scaffolds and after 10 load cycles.DNA content (A) and total sGAG content (sample + medium) (B) of MSCs in loaded scaffolds distinguished by counterface type (Ball and Cylinder) or unloaded (UL) condition and loading protocols. Data are shown as mean ± SEM of three independent experiments with cells from three individual donors. Each experimental group was run in technical replicates. Statistical testing was not performed on single conditions but on contrasts (Supp. Fig. 2). s shear, c compression.