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. 2022 Mar 25;7:85. doi: 10.1038/s41392-022-00898-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Functional comparison of CD5/CD7 bispecific CAR-T cells was performed in vitro. a CD5/CD7 bispecific CAR-T cells specifically kill CD5⁺ and/or CD7⁺ tumor cells. The data indicate mean ± SD (three replicates). b Expansion of indicated CAR-T and T cells during multi-rounds of tumor cells stimulation. The data represent mean ± SD from three donors. The X-axis represents different coculture time points of CAR-T/T cells with CCRF-CEM cells and the arrows mean mitomycin C-treated CCRF-CEM cells were added at indicated time. c Cytolysis assay of CAR-T/T cells after two rounds of stimulation by mitomycin C-treated CCRF-CEM cells. CAR-T/T cells were remixed with tumor cells at indicated ratios (CAR⁺% to tumor cells) to evaluate the cytotoxicity by luciferase-based assay. The data represent mean ± SD (n = 3). d CAR-T cells were stained with antibodies against LAG-3, TIGIT, and TIM-3, respectively, before and after two stimulations by mitomycin C-treated CCRF-CEM. The results are displayed as mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, two-way ANOVA