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. 2022 Mar 24;13:1594. doi: 10.1038/s41467-022-29244-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Schematic depicting the location of AVP and VIP neurons in the SCN. b, c, m Photomicrographs of VIP (b), AVP (c), and mPER2 (m) immunoreactivity in the SCN of control and Ubr4 cKO mice at defined CTs. Scale bar, 100 μm. d–f Immunoreactive intensity of VIP in the whole (d), core (e), or shell (f) SCN. g, h Density of VIP-IR cells in the SCN at CT 2 (g) or CT 14 (h) as a function of expression intensity. i, j Immunoreactive intensity of AVP in the whole SCN (i) or in the soma of shell SCN neurons (j). k, l Density of AVP-IR cells in the SCN at CT 2 (k) or CT 14 (l) as a function of expression intensity. Low threshold values (on the x-axis in g, h, k, l) indicate strong expression, whereas high threshold values indicate weak expression. n = 4 control (CT 2 and CT 14), 4 Ubr4 cKO (CT 2), 5 Ubr4 cKO (CT 14) (d–l). *p = 0.048, ***p < 0.0001 (e); *p = 0.0449 (CT 2), *p = 0.0159 (CT 14) (f); **p = 0.0058, ***p = 0.0002 (g); *p = 0.0378, **p = 0.0052 (110), **p = 0.0044 (120) (h); **p = 0.0037, ***p = 0.0001 (j); *p = 0.0329 (100), *p = 0.0338 (110) (k); *p = 0.0438, **p = 0.008 (l). n Immunoreactive intensity of mPER2 in the whole SCN. n = 8 control and 4 Ubr4 cKO; **p = 0.0083, ***p = 0.0005. o Density of mPER2-IR cells in the SCN. In ascending order of CT: n = 8, 8, 7, 8, 7, 7 for control; n = 4, 3, 4, 4, 4, 4 for Ubr4 cKO. *p = 0.0233, ***p < 0.0001. Values represent mean ± SEM. “n” represents the number of mice. Two-way ANOVA with Bonferroni’s post hoc. A.U. arbitrary units.