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. 2022 Mar 24;13:1594. doi: 10.1038/s41467-022-29244-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a PDF immunoreactivity (red) in the whole fly brain, optic lobe (OL), dorsal projections (DP), dorsal terminals (DT), posterior optic tract (POT), and large (l-LN_v) and small (s-LN_v) ventral lateral neurons at CT 2 and CT 14. Scale bar, 60 μm (whole brain), 30 μm (OL, POT, DP, DT), and 10 μm (l-LN_v, s-LN_v). b–g PDF-IR intensity in the OL (b), POT (c), DP (d), DT (e), and the soma of l-LN_vs (f) or s-LN_vs (g). n = 15 (b–e), 14 (f), or 11 (g) for +>poe^RNAi (CT 2); n = 10 (b–e) or 4 (f, g) for +>poe^RNAi (CT 14). n = 13 (b, c), 12 (d, e), or 10 (f, g) for Pdf>Dcr2 (CT 2); n = 7 (b, g), 8 (c–e), or 6 (f) for Pdf>Dcr2 (CT 14). n = 16 (b), 15 (c–e), or 11 (f, g) for Pdf>Dcr2; poe^RNAi (CT 2); n = 8 (b, c, e–g) or 7 (d) for Pdf>Dcr2; poe^RNAi (CT 14). ***p < 0.0001 (b, d, e); *p = 0.0385, ***p < 0.0001 (CT 2), ***p = 0.0006 (CT 14) (c); *p = 0.0211, **p = 0.0058, ***p = 0.0001 (CT 2), ***p = 0.0005 (CT 14) (g). h, i dPER (magenta) and PDF (green) immunoreactivity in s-LN_vs (h) and l-LN_vs (i) under DD. Scale bar, 10 μm. j, k d-PER-IR intensity in s-LN_vs (j) and l-LN_vs (k) as a function of CT. In ascending order of CT: n = 6, 10, 10, 8, 4, 7, 6, 8 for +>poe^RNAi (j); n = 6, 11, 8, 8, 4, 7, 5, 9 for +>poe^RNAi (k); n = 5, 9, 5, 9, 4, 8, 5, 9 for Pdf>Dcr2 (j, k); n = 7, 11, 10, 8, 4, 7, 6, 7 for Pdf>Dcr2; poe^RNAi (j); n = 7, 11, 10, 9, 3, 7, 7, 7 for Pdf>Dcr2; poe^RNAi (k). *p = 0.0485, **p = 0.0017 (CT 8), **p = 0.0054 (CT 17), ***p < 0.0001 (j). Values represent mean ± SEM. “n” represents number of flies. Two-way ANOVA with Bonferroni’s post hoc. A.U. arbitrary units.