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. 2022 Mar 24;12:5073. doi: 10.1038/s41598-022-08978-4

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© This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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MR has sex-specific effects on physiological measures. Body weight (a), (b) and normalized food consumption (g), (h) measured weekly while on MR diet. Body composition data for fat mass (c), (d), lean mass (e), (f) measured every two weeks. Sexes analyzed separately with Repeated Measures, 3-Way ANOVA. Main effects for diet and MsrA KO genotype indicated by significance bars by legends (right side and above, respectively). Post-hoc diet specific effect within a genotype was analyzed with Repeated Measures, 2-Way ANOVA Multiple Comparisons with False Discovery Rate correction, Q = 0.05. Means at specific time points being significantly different (p < 0.05) between diets for wild type denoted by dollar sign ($) and for MsrA KO denoted by a pound sign (#). Graphs represent means ± s.e.m, and all groups were 8–10 mice. (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001).