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. 2022 Mar 24;12:5130. doi: 10.1038/s41598-022-09051-w

Figure 2.

Figure 2

Both siGGCAGU and siGGGGGC are toxic to cancer cells. (A) Change in confluence (growth) over time of HeyA8 cells reverse transfected with either 1 or 10 nM of siNT1, siGGCAGU, or siGGGGGC. ANOVA p-values between the two toxic siRNAs are shown. ns not significant. Each data point represents mean ± SE of six replicates. (B) Percent viability (ATP content) 96 h after transfecting HeyA8 cells (forward or reverse) with 1 or 10 nM of the indicated siRNAs, or with lipid only or left untreated. Experiment was done in 6 replicates. ***p < 0.0001, **p < 0.001. (C) Phase contrast images showing morphology of HeyA8 cells reverse transfected in A at 10 nM 4 days post transfection. (D) Change in confluence over time of HCT116 (human colon cancer) and H4 (human neuroglioma), PC3 (human prostate cancer), or GL261 (mouse glioma) cells reverse transfected with 10 nM (HCT116, H4, and PC3) or 25 nM (GL261) of siNT1, siGGCAGU, or siGGGGGC. Each data point represents mean ± SE of six or four replicates, respectively. In all three cases the optimal amount of transfection reagent (lipid) was used to avoid non-specific growth inhibition.