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. 2022 Mar 24;12:5096. doi: 10.1038/s41598-022-08940-4

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a–d) Microscopic images ( $5 \times$ magnification) of the H&E-stained biopsies from the healthy skin (control experiment) and three representative burns created using 70 $^{\circ}$ , 80 $^{\circ}$ , and 98 $^{\circ}$ scald, respectively, the blue and black arrows indicate the areas of collagen denaturation and vascular occlusion, respectively, (e) the dermal burn percentage measured on the biopsies on Day 0, and (f) Day 4.