Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Mar 24;12:5132. doi: 10.1038/s41598-022-09241-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mechanistic effects of LAS194046 improving corticosteroids anti-inflammatory effects. Neutrophils from (A) COPD and (B) severe asthma patients were pre-incubated with sub-effective concentrations of LAS194046, fluticasone propionate or its combination during 1 h followed by cell stimulation with LPS (1 µg/ml) for 6 h. MKP1 and PI3Kδ gene expression and PI3Kδ activity were measured. The results are expressed as the mean ± SD (n = 3 each for cells from severe asthma and COPD patients in independent experiments with triplicate samples). A two-way ANOVA was followed by a post hoc Bonferroni test. *p < 0.05 vs. control; ^#p < 0.05 vs LPS. (C) Bronchial epithelial Beas2B cells were transfected with a GRE reporter gene and stimulated with different combinations of LAS194046 and fluticasone propionate. The results are expressed as the mean ± SD (n = 4 run in triplicate). A one-way ANOVA was followed by a post hoc Bonferroni test. *p < 0.05 vs. LAS194046 monotherapy; ^#p < 0.05 vs. fluticasone propionate monotherapy.