Table 4.
Primer sequences utilized for rRT-PCR analysis of targeted gene expression.
| Target gene | Primer sequence (5′–3′) | Accession No./Reference |
|---|---|---|
| iNOS |
F-ACCTTCCGGGCAGCCTGTGA R-CAAGGAGGGTGGTGCGGCTG-3′ |
NM_012611 |
| COX-2 |
F-GCTCAGCC ATACAGCAAATCC R-GGGAGTCGGGCAAT CATCAG |
NM_017232 |
| Caspase-3 |
F-GCAGCTAACCTCAGAGAGACATTC R-ACGAGTAAGGTCATTTTTATTCCTGACTT |
NM_012922 |
| Bcl-2 |
F-TGCGCTCAGCCCTGTG R-GGTAGCGACGAGAGAAGTCATC |
NM_016993 |
| BAX |
F-CAAGAAGCTGAGCGAGTGTCT R-CAATCATCCTCTGCAGCTCCATATT |
NM_017059 |
| Cytochrome C |
F-TTTGAATTCCTCATTAGTAGCTTTTTTGG R-CCATCCCTACGCATCCTTTAC |
NM_012839 |
| IL-1β |
F-TGACAGACCCCAAAAGATTAAGG R-CTCATCTGGACAGCCCAAGTC |
NM_031512.2 |
| IL-6 |
F-CCACCAGGAACGAAAGTCAAC R-TTGCGGAGAGAAACTTCATAGCT |
NM_012589.2 |
| IL-10 |
F-GCCCAGAAATCAAGGAGCATT R-CAGCTGTATCCAGAGGGTCTTCA |
L02926.1 |
| TNFα |
F-CAGCCGATTTGCCATTTCA R-AGGGCTCTTGATGGCAGAGA |
L19123.1 |
| TGF-β |
F-CCAGCCGCGGGACTCT R-TTCCGTTTCACCAGCTCCAT |
NM_021578.2 |
| β-actin |
F-CGCAGTTGGTTGGAGCAAA R-ACAATCAAAGTCCTCAGCCACAT |
V01217.1 |
| GAPDH |
F-TGCTGGTGCTGAGTATGTCG-3′ R-TTGAGAGCAATGCCAGCC-3′ |
NM_017008 |
| Bacteroides spp. |
F:GAG AGG AAG GTC CCC CAC R:CGC TAC TTG GCT GGT TCA G |
Layton et al.94 |
| Firmicutes spp. Guo et al. 2008 |
F-GGAGYATGTGGTTTAATTCGAAGCA R-AGCTGACGACAACCATGCAC |
Guo et al.95 |
| Enterobacteriaceae |
F-CATTGACGTTACCCGCAGAAGAAGC R-CTCTACGAGACTCAAGCTTGC |
Bartosch et al.96 |
| Bifidobacterium spp. |
F-GCG TCC GCT GTG GGC R-CTT CTC CGG CATGGT GTTG |
Requena et al.97 |
| Bacillus spp. |
F-GCA ACG AGC GCA ACC CTT GA R-TCA TCC CCA CCT TCC TCC GGT |
Zhang et al.37 |
iNOS inducible nitric oxide synthase, COX-2 cyclo-oxygenase-2, IL interleukin, TNFα tumor necrosis factor α, TGF-β transforming growth factor-beta, COX-2 cyclooxygenase-2, Bcl-2 B cell lymphoma-2, Bax Bcl-2 associated x protein.