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. 2022 Mar 24;8:40. doi: 10.1038/s41523-022-00402-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, b Representative western blot results showing that the effects of SFN at different concentrations (7.5, 15, and 30 μM for MDA-MB-231 cells and 3.8, 7.5, and 15 μM for MDA-MB-157 cells) on TGF-β1-induced actin stress fiber formation-associated protein expression, including paxillin, focal adhesion kinase (FAK), Ras GTPase-activating-like protein (IQGAP1), Rho-kinase (ROCK), and p21-activated kinase 2 (PAK2) in MDA-MB-231 (a) and MDA-MB-157 (b) cells. c, d Statistical assay showing that the effects of SFN at different concentrations on TGF-β1-induced actin stress fiber formation-associated proteins expression in MDA-MB-231 (c) and MDA-MB-157 (d) cells. Data shown are mean ± SEM. n = 3, ^∗P < 0.05 vs Cont; ^#P < 0.01 vs TGF-β1.