Fig. 7.

GGPPS1 knockdown ameliorated cytokine release from RAW264.7 cells via regulation of Rab37-mediated autophagy. a RAW264.7 cells transfected with GGPPS1-specific shRNA (shGGPPS1) or negative control shRNA (shNC) were subjected to cyclic stretch (CS) or not. Cells were treated with RAPA to induce autophagy. The content of inflammatory cytokines in the cell supernatant was analyzed by ELISA. b Protein expression of cytokines was analyzed by western blot. Rab37 protein expression levels in c GGPPS1^−/− mice subjected to mechanical ventilation (VT) and d GGPPS1 knockdown RAW264.7 cells subjected to CS were analyzed by western blot. e The Rab37 overexpression plasmid (pcRab37) was transfected into RAW264.7 cells. The content of inflammatory cytokines in the cell supernatant was analyzed by ELISA. f Protein expression of cytokines was analyzed by western blot. *P < 0.05; **P < 0.01; ***P < 0.001 vs. the indicated group