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Mutation of two D14 genes in Nicotiana benthamiana with CRISPR‐Cas9. (a) Diagram of D14a and D14b genes in N. benthamiana. Blue boxes represent exons. Vertical red lines indicate gRNA target sites. (b) Sequences of Cas9‐induced frameshift alleles of NbD14a and NbD14b. gRNA target sequence is underlined. Protospacer adjacent motif (PAM) sequence (5′‐NGG‐3′) is indicated in bold blue font. Red triangles denote predicted Cas9 cleavage sites
