FIGURE 5.

AtSMXL7 degradation is altered when coexpressed with AtD14 variants. Background‐corrected AtSMXL7‐mScarlet‐I to Venus fluorescence in Nbd14a,b leaf discs after 16‐h treatment with solvent control (0.025% acetone [v/v]), 2‐, 10‐, 50‐, or 250‐nM rac‐GR24. pRATIO3212‐AtSMXL7 was coexpressed with pGWB415‐AtD14 or (a) pGWB415‐AtD14^F136A, (b) pGWB415‐AtD14^K243I, (c) pGWB415‐AtD14^F136A/K243I, or (d) pGWB415‐AtD14^D167A. Box plots show median with 25th and 75th percentiles, and Tukeys whiskers (n = 6–8 independently transformed leaves, where each leaf value is the mean value of 3–6 discs). *p < .05, repeated‐measures two‐way ANOVA with Dunnetts multiple comparisons test, comparing GR24 treatments with control for each genotype. Percentages below box plots indicate the percent change in the mean mScarlet‐I/Venus ratio of each treatment compared with control