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. 2022 Jan 22;9(9):2105568. doi: 10.1002/advs.202105568

Figure 4.

Figure 4

cAMP activation boosted thyroid follicles maturation for hormone secretion in hFTOs. A) Representative bright‐field images of organoids at indicated days during primary culture at growth‐factor (GF) medium and forskolin treatment (+Fors.) condition. Scale bar = 100 µm. B) Hematoxylin and eosin staining on organoids indicated in (A), scale bar = 50µm. C) Immunofluorescence double‐labeled thyroid organoid of ZO‐1 and TG, and nuclei staining with DAPI. Scale bar = 50 µm. D) Quantification of follicle perimeter in organoids that are cultured in growth‐factor (GF) medium and forskolin treatment (+Fors.) condition. Data are represented as mean ± SD, adjusted p‐value (Unpaired t‐test) p < 0.0001. n = 87 for GF condition and n = 63 for Forskolin treatment condition. E) qRT‐PCR analysis of gene expression of thyrocyte markers and cAMP signaling related genes. F) Free T4 secretion measured after 4 days culturing in growth‐factor (GF) medium and forskolin treatment (+Fors.) condition. Data are represented as mean ± SD in two independent experiments.