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. 2022 Jan 17;9(9):2105587. doi: 10.1002/advs.202105587

Figure 1.

Figure 1

ADF promotes hepatic metabolic remodeling with disrupted complex II assembly. a) Glucose tolerance test and b) insulin tolerance test in mice under regular feeding or ADF intervention for 4 weeks, n = 8. c) Metabolomics analysis for liver of mice under regular feeding or ADF intervention for 4 weeks, with Z‐score plot of global metabolic profiles among all the samples and d) metabolic pathway enrichment analysis, n = 8. e) Mitochondrial electron transport chain complex activities in liver, n = 8. f) Complex II activity in skeletal muscle, inguinal white adipose tissue (iWAT), and brown adipose tissue (BAT), n = 8. g) Immunoblots analysis for complex II subunits and assembly factors SDHAF2 and SDHAF4 in liver, representative blotting images, h) summary analysis of arbitrary unit. i) mRNA levels of representative mitochondrial complex subunits in liver, n = 8. j–m) Coimmunoprecipitation of j) SDHAF2 with SDHA/SDHB, k) SDHAF4 with SDHA/SDHB, l) SDHA with SDHB, and m) SDHB with SDHA from the lysates of mice liver after ADF intervention. Values are mean ± SEM, *p < 0.05, **p < 0.01.