Figure 2.

Hepatic Sdhaf4 knockout mice maintain normal phenotype with disrupted SDH complex. The control and Sdhaf4 ^Alb KO mice were analyzed at the age of 16 weeks: a) Representative images of tissues and summary analysis of body and tissue weight, n = 8. b) Immunoblots for complex II subunits in liver of control and Sdhaf4 ^Alb KO mice, representative blotting images, c) summary analysis of arbitrary unit, n = 8. d,e) Immunoprecipitation of ubiquitin for blot analysis of SDHA and SDHB from liver lysates of control and Sdhaf4 ^Alb KO mice, n = 3. f–h) Co‐immunoprecipitation of SDHA with SDHB, SDHB with SDHA, for analysis of SDHA/SDHB binding from liver lysates of control and Sdhaf4 ^Alb KO mice, n = 3. i,j) HE staining and transmission electron microscope (TEM) analysis of liver section in control and Sdhaf4 ^Alb KO mice, i) representative images, j) analysis of mitochondrial per area. k) Metabolic cage analysis of control and Sdhaf4 ^Alb KO mice, locomotor activity, l) whole body oxygen consumption rate, m) CO₂ production rate, and n) heat generation, n = 8. Values are mean ± SEM, *p < 0.05, **p < 0.01.