Figure 5.

GPX3 is the direct target of miR-665. (a) The complementary sequences between miR-665 and GPX3 were shown. (b) Dual-luciferase reporter assays were performed to confirm the association between miR-665 and GPX3 3′UTR. (c, e) The expression of GPX3 in NSCLC tissues (n = 40) and cells was tested by qRT-PCR. (d, f) Western blot examined the protein level of GPX3 in NSCLC tissues (n = 40) and cells. (g) Western blot was used to detect the expression of GPX3 in transfected A549 and HCC827 cells. ∗∗∗P < 0.001.