Table 2.

I-optimal Design of BGT-loaded NSPs with their responses.

Formula Code	Sonication Time (min.)	Type of EA	Span-60:EA Ratio	EE (%)	Vesicles Size (nm)	Zeta Potential (mV)	PDIa
S1	5	Tween® 80	4:1	88.7 ± 1.31	465.7 ± 11.45	−23.5 ± 1.03	0.441 ± 0.12
S2	10	Pluronic F127	3:2	45.8 ± 2.37	773.4 ± 15.46	−28.2 ± 0.74	0.627 ± 0.02
S3	5	Tween® 80	3:2	72.8 ± 1.76	493.5 ± 14.32	−22.5 ± 2.31	0.573 ± 0.06
S4	7.5	Tween® 80	3:2	68.3 ± 3.57	460.2 ± 8.56	−26.2 ± 1.76	0.351 ± 0.14
S5	5	Pluronic F127	4:1	63.5 ± 1.89	621.6 ± 22.47	−24.3 ± 1.45	0.432 ± 0.15
S6	7.5	Pluronic F127	3:2	47.3 ± 2.67	794.3 ± 13.45	−25.4 ± 0.83	0.631 ± 0.05
S7	5	Pluronic F127	3:2	52.3 ± 2.58	832.2 ± 23.34	−22.4 ± 1.48	0.507 ± 0.12
S8	7.5	Pluronic F127	4:1	56.2 ± 2.54	592.4 ± 21.76	−29.3 ± 2.43	0.235 ± 0.10
S9	7.5	Tween® 80	4:1	83.1 ± 1.45	402.6 ± 16.54	−24.6 ± 1.98	0.436 ± 0.09
S10	7.5	Pluronic F127	4:1	54.5 ± 1.96	589.3 ± 18.43	−30.2 ± 2.45	0.602 ± 0.11
S11	10	Pluronic F127	4:1	54.7 ± 2.03	579.4 ± 25.43	−33.2 ± 1.73	0.648 ± 0.07
S12	10	Tween® 80	3:2	65.7 ± 1.94	441.3 ± 17.65	−32.8 ± 2.45	0.553 ± 0.1
S13	10	Tween® 80	4:1	81.5 ± 2.57	388 ± 8.93	−29.6 ± 1.85	0.474 ± 0.2