Figure 10.

Real-time RT-PCR analyses: (A) Tail suspension was performed for two weeks using male wild-type and Sp7 tg mice. The expression of Sp7, Runx2, Spp1, Ibsp, Col1a1, Bglap & Bglap2, Rankl, and Opg, and the Rankl/Opg ratio. The number of mice that were analyzed; grounded-loaded wild-type: 14, unloaded wild-type: 6, grounded-loaded tg: 11, unloaded tg: 9. (B) Tail suspension was performed for three days using male C57BL/6 mice. The number of mice that were analyzed; grounded-loaded wild-type: 8, unloaded wild-type: 7. (C) Tail suspension was performed for one week using male C57BL/6 mice. The number of mice that were analyzed; grounded-loaded wild-type: 9; unloaded wild-type: 9. In (A–C), the values in the grounded-loaded wild-type mice were defined as one, and relative levels are shown. *^,# p < 0.05, **^,## p < 0.01, ### p < 0.001 by Tukey–Kramer test * and Student’s t-test #.