Cleaved caspase-3 and PARP in tumor tissues analyzed by immunohistochemistry (IHC). After 19 days of treatment with heat-killed single strains or their mixtures, tumor tissues were harvested and stained by IHC. (a) Cleaved caspase-3, (b) cleaved PARP. Black arrows indicate the dots of cleaved caspase-3 and cleaved PARP (magnification 20× or 40× as indicated). The scale bar represents 200 px. Control, drinking water; MG731, B. bifidum MG731 (1 × 109 cells/mouse); MG5346, L. reuteri MG5346 (1 × 109 cells/mouse); MG4584, L. casei MG4584 (1 × 109 cells/mouse); 2Mix, mixture of L. reuteri MG5346 (1 × 109 cells/mouse) and L. casei MG4584 (1 × 109 cells/mouse); 3Mix, mixture of B. bifidum MG731 (1 × 109 cells/mouse), L. reuteri MG5346 (1 × 109 cells/mouse), and L. casei MG4584 (1 × 109 cells/mouse).