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. 2022 Mar 15;14(6):1243. doi: 10.3390/nu14061243

Figure 6.

Figure 6

Western blot and immunohistochemistry of muscarinic acetylcholine receptor subtype 3 (mAChR3). Samples of frontal cortex (A) and hippocampus (C) from rats fed with standard diet (CHOW rats) and high-fat diet (DIO rats) for 5 and 17 weeks were immunoblotted using specific anti- mAChR3. Graphs report the densitometric data with CHOW rats as control, and β-actin was used as a reference loading protein. Data are mean ± S.E.M. CHOW rats 5 weeks n = 8; DIO rats 5 weeks n = 8; CHOW rats 17 weeks n = 8; DIO rats 17 weeks n = 9. Representative pictures of CHOW and DIO rats in frontal cortex after 5 weeks (B) and hippocampus, CA3 subfield, after 17 weeks (D) of diet. V, fifth layer of frontal cortex; O, stratum oriens; P, pyramidal neurons; R, stratum radiatum. Scale bar: 25 µm.