Table 1.
Primary antibodies used in immunohistochemistry (IHC) and Western blot (WB).
| Antibody | Company and Cat. No | Dilution IHC |
Dilution WB |
|---|---|---|---|
| Neuronal nuclei (NeuN) |
Merck Millipore Cat. MAB377 |
1:500 | 1:1000 |
| Neurofilament 200 kDa (NF) |
Merck Millipore Cat. MAB526 |
1:500 | 1:1000 |
| Choline acetyltransferase (ChAT) |
Chemicon/Millipore Cat. AB144P |
1:50 | 1:150 |
| Acetylcholinesterase (AChE) |
Santa Cruz Biotechnology Cat. sc6430 |
1:100 | 1:500 |
| Vesicular acetylcholine transporter (VAChT) |
Santa Cruz Biotechnology Cat. sc7717 |
1:100 | 1:500 |
| Alpha7 nicotinic acetylcholine receptor (α7nAChR) | Santa Cruz Biotechnology Cat. sc5544 |
1:50 | 1:500 |
| Muscarinic acetylcholine receptor subtype 1 (mAChR1) |
Santa Cruz Biotechnology Cat. sc9106 |
1:50 | 1:500 |
| Muscarinic acetylcholine receptor subtype 3 (mAChR3) |
Santa Cruz Biotechnology Cat. sc7474 |
1:50 | 1:500 |
| Muscarinic acetylcholine receptor subtype 5 (mAChR5) |
Santa Cruz Biotechnology Cat. sc7479 |
1:50 | 1:500 |
| Beta-actin (β-actin) | Sigma-Aldrich Cat. A2228 |
/ | 1:3000 |
| Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) |
Sigma-Aldrich Cat. G9295 |
/ | 1:5000 |