Figure 10.

The time-dependent internalization (confocal microscopy) and intracellular fate of Lip^DOPE-siRNA in keratinocytes (HaCaT) cell culture. Cells were treated with 6 µg/mL (siRNA concentration) and examined for siRNA endosomal/lysosomal escape. The endo-lysosomes are depicted in red (LysoTracker Red DND-99), fluorescently-labeled siRNA (siFAM) is depicted in green, and colocalization is depicted in yellow (white arrows). The fluorescence intensity was normalized to untreated cells; magnification ×60 (scale bar = 50 μm).