Table 4.
Linearity, matrix effect and limits for atropine and scopolamine analysis in thyme (Thy), basil (Ba) and coriander (Cor) samples with the optimized SLE-SPE-HPLC-MS/MS method.
| Atropine | Scopolamine | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Sample | Linearity (µg/mL) | Matrix Matched Calibration |
R2 | MDL a (µg/kg) |
MQL b (µg/kg) |
ME c (%) |
Matrix Matched Calibration |
R2 | MDL a (µg/kg) |
MQL b (µg/kg) |
ME c (%) |
| Thy | 0.005–0.2 | 5.8 × 108 x + 9.9 × 105 | 0.999 | 0.8 | 2.6 | −36 | 2.0 × 108 x + 3.4 × 105 | 1.000 | 1.2 | 4.0 | −43 |
| Ba | 0.005–0.2 | 4.5 × 108 x + 9.5 × 105 | 0.999 | 1.6 | 5.3 | −50 | 1.4 × 108 x + 2.1 × 105 | 0.999 | 1.3 | 4.4 | −59 |
| Cor | 0.01–0.2 | 3.6 × 108 x − 1.7 × 106 | 0.999 | 2.1 | 6.8 | −61 | 1.0 × 108 x − 6.5 × 105 | 0.999 | 2.2 | 7.2 | −71 |
a MDL: method detection limit. b MQL: method quantification limit. c ME: matrix effect. To estimate ME, solvent-based calibration was prepared between 0.005–10 µg/mL (At: y = 9.1 × 108 x + 5.8 × 107; Sc: y = 3.5 × 108 x + 5.5 × 107).