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. 2022 Mar 16;14(3):649. doi: 10.3390/pharmaceutics14030649

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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2-DG uptake by HSkMCs with and without CB treatment. (A) Comparison of insulin and hUC-MSCs-sEVs on stimulation of 2-DG uptake by HSkMCs. Treatment of hUC-MSCs-sEVs at 20 µg/mL showed a significant increase in 2-DG uptake by HSkMCs. (B) Effect of hUC-MSCs-sEVs on 2-DG uptake by cytochalasin B-treated HSkMCs. Cytochalasin B treatment at 0.5 µM significantly reduced the 2-DG uptake in HSkMCs. However, hUC-MSCs-sEVs seems unable to reverse the action of cytochalasin B. Data are expressed as the mean ± SD of at least three independent assays with triplicate samples in each assay (* p < 0.05, ** p < 0.01, *** p < 0.001, one-way ANOVA with Tukey post hoc tests).