Skip to main content
. 2022 Mar 12;23(6):3065. doi: 10.3390/ijms23063065

Figure 2.

Figure 2

Linagliptin suppresses TNF-α-induced tissue factor expression via an Akt/eNOS pathway. HUVECs were pretreated with different doses (1 and 10 µM) of linagliptin for 48h and then incubated with 0.5 ng TNF-α for 2h. Gene expression and protein levels were detected by qPCR and western blot. (A) Expression levels of tissue factor (TF). (B) Phosphorylation ratio of eNOS. (C) Phosphorylation ratio of Akt. (D) Protein levels of TF with or without L-NAME pretreatment, an eNOS inhibitor. n = 6 in each group. + p ≤ 0.05 compared to respective control. * p ≤ 0.05 compared to TNF-α-treated cells. # p ≤ 0.05 compared to TNF-a plus linagliptin-treated group.