Table 3.
ANOVA of best fitted designing model for vesicle size (Y1) and entrapment efficiency (Y2).
| Vesicle Size (VS) | |||||
|---|---|---|---|---|---|
| Source | Sum of Squares | Mean Square | F-Value | p-Value Prob > F | Remark |
| Model (2nd order) | 74,666.22 | 18,666.55 | 646.03 | <0.0001 | Significant |
| A-CHO | 29,058.51 | 29,058.51 | 1005.69 | <0.0001 | -- |
| B-Lipid | 30,284.67 | 30,284.67 | 1048.13 | <0.0001 | -- |
| C-Surfactant | 11,128.26 | 11,128.26 | 385.14 | <0.0001 | -- |
| D-Bile salt | 4194.78 | 4194.784 | 145.17 | <0.0001 | -- |
| Residual | 635.66 | 28.89394 | -- | -- | -- |
| Lack of Fit | 631.00 | 31.55001 | 13.52 | 0.0710 | NS |
| Pure Error | 4.66 | 2.333333 | -- | -- | -- |
| Total | 75,301.89 | -- | -- | -- | -- |
| Entrapment efficiency (EE) | |||||
| Model | 3599.88 | 359.98 | 36.53 | <0.0001 | Significant |
| A-CHO | 1779.01 | 1779.01 | 180.54 | <0.0001 | -- |
| B-Lipid | 778.43 | 778.43 | 79.00 | <0.0001 | -- |
| C-Surfactant | 544.05 | 544.05 | 55.21 | <0.0001 | -- |
| D-Bile salt | 239.41 | 239.41 | 24.29 | 0.0002 | -- |
| AB | 110.25 | 110.25 | 11.18 | 0.0041 | -- |
| AC | 0.66 | 0.66 | 0.06 | 0.7985 | -- |
| AD | 6.25 | 6.25 | 0.63 | 0.4374 | -- |
| BC | 1.22 | 1.22 | 0.12 | 0.7294 | -- |
| BD | 91.58 | 91.58 | 9.29 | 0.0077 | -- |
| CD | 49 | 49 | 4.97 | 0.0404 | -- |
| Residual | 157.65 | 9.85 | -- | -- | -- |
| Lack of Fit | 155.44 | 11.10 | 10.04 | 0.0942 | NS |
| Pure Error | 2.21 | 1.105 | -- | -- | -- |
| Total | 3757.54 | -- | -- | -- | -- |