Table 3.

Primers used in the present study, detailing its parameters used in the PCR protocol and the expected size of band for the PCR product.

Gene	Primer	Sequence	To (°C) †	Expected Length (bp)	Reference
COI	Cox1_schis’_5′	TCTTTRGATCATAAGCG	* 50–46 phase 1; 45 phase 2	1000	Lockyer et al. [46]
	Cox1_schis’_3′	TAATGCATMGGAAAAAAACA
	CO1F15	TTTNTYTCTTTRGATCATAAGC	* 50–46 phase 1; 45 phase 2	600	Brant & Loker [2]
	CO1R15	TGAGCWAYHACAAAYCAHGTATC
	CO1RH3R internal	TAAACCTCAGGATGCCCAAAAAA
28S	U178	GCACCCGCTGAAYTTAAG	* 55–51 phase 1; 50 phase 2	1500	Lockyer et al. [46], Tkach et al. [47], Olson et al. [48]
	L1642	CCAGCGCCATCCATTTTCA
	DIG12 internal	AAGCATATCACTAAGCGG
	ECD2 internal	CTTGGTCCGTGTTTCAAGACGGG

† Observable temperature; * Touchdown PCR. The annealing temperature was programmed to reduce to one degree per cycle (50–46 °C; 55–51 °C) for 15 cycles in phase one and a total of 20 cycles in phase two.