TABLE 2.
TK activity of VZV strains
| Strain | Activity (mean ± SD) (pmol/mg of protein/h)a of:
|
|
|---|---|---|
| [3H]thymidineb | [3H]araTc | |
| Noninfected HeLa cells | 217 ± 57 | 0 |
| VZV strains | ||
| Ellen | 13,472 ± 478 | 4,208 ± 643 |
| Oka | 12,868 ± 926 | 2,210 ± 89 |
| D9507 | 12,619 ± 1,642 | 2,737 ± 1,450 |
| WMad | 3,182 ± 619 | 485 ± 44 |
| WStr | 303 ± 169 | 0 |
| LYON6625 | 156 ± 49 | 0 |
| SW689 | 103 ± 41 | 0 |
a
Data were calculated from at least three independent assays as picomoles of the nucleoside analogue phosphorylated in 1 h per mg of protein at 37°C.
b
Final concentration of thymidine, 25 μM. Resulting specific activity in the reaction mixture, 1 Ci/mmol.
c
Final concentration of araT, 8.1 μM. Resulting specific activity in the reaction mixture, 3 Ci/mmol.