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. 2022 Mar 8;23(6):2914. doi: 10.3390/ijms23062914

Figure 3.

Figure 3

Effect of Luteolin-7-O-glucoside (Lut7) on SH-SY5Y cells after exposition to 6-OHDA (100 µM) over 6 h. (A) Effect on Caspase-3 activity (Δ Fluorescence (U.A)/mg of protein/min); (B) ROS production; (C) MMP (ratio of monomers/aggregates of JC-1); and (D) AChE activity (nmol/min/mg of protein). The values in each column represent the mean ± standard error of the mean (SEM) of at least 3 independent experiments carried out in triplicate. Statistical calculations were performed with ANOVA via the Tukey post hoc test and significant differences were considered for *** p< 0.001 (vs. control); ### p < 0.001 and ## p < 0.01 (vs. 6-OHDA). Negative control (untreated cells) was considered to be 100% viable and is represented by the red dashed line. DMSO 0.1% was used as a vehicle.