Figure 1.

Concentration dependence of insulin oligomerisation state in the refolding buffer monitored by light scattering: (a) Scattered light intensities at 90° angle from solutions of insulin protein at different concentrations expressed in terms of the Rayleigh ratio R_90° normalised by (KcM₀) values; (b) second-order autocorrelation functions g₂ (τ) from dynamic light scattering experiment carried out on insulin at 2 mg/mL. The continuous line represents the fitting curve resulting from data analysis by the method of cumulants.