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Non-denaturant PAGE of insulin at 0.1 and 0.5 mg/mL with and without GroEL/GroES in a molar ratio GroEL:insulin = 1:10. In addition, GroEL at 0.1 mg/mL (which corresponds to the concentration of GroEL incubated with 0.1 mg/mL insulin at a molar ratio chaperonin/insulin 1:10) and GroEL/GroES mix (GroEL:GroES = 1:1 with GroEL at 0.1 mg/mL) were loaded. The original non-denaturant page is reported in the Supplementary Materials (Figure S1).
