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. 2022 Mar 6;10(3):569. doi: 10.3390/microorganisms10030569

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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(A). Population growth rate in the presence (+) or absence (−) of combined nitrogen for nif-containing strains MU08 and MU09 and for nif-lacking strain CCMEE 5410. Error bars are standard errors. (B). Maximum likelihood phylogenies for nifHDK and 16S rRNA genes reconstructed with a GTR+I+G model. Bootstrap support values greater than 50% are shown for 1000 bootstrap replicates. Branch lengths are in units of expected number of nucleotide substitutions per site. The two topologies were significantly different by an SH test (p < 0.0001).