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. 2022 Mar 18;11(6):863. doi: 10.3390/foods11060863

Figure 2.

Figure 2

Optimization of the EuNP-FLFIA assay. (A) The relationship between detection time and the T/C value. The sample concentration was 0, 0.5, and 1 ng/mL, respectively. (B) Effect of buffers on the T/C value. Buffer types: ultra-pure water, BBS buffer, PBS buffer, and CBS buffer. (C) Effect of different EuNP polyclonal antibody probe concentrations on the fluorescence intensity of the mixed solutions. (D) The fluorescence intensity values and inhibition intensity are shown by using different nitrocellulose membranes. Nitrocellulose membrane types: M70, HF90, M110, HF135, CN140, and HF180. (E) Effect of EuNP polyclonal antibody probe concentrations on the fluorescence intensity. (F) Influence of various CLE-BSA-RAC concentrations and EuNP polyclonal antibody probe concentrations on the T/C value.