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. 2022 Feb 28;12(3):351. doi: 10.3390/life12030351

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Suppression of GCS reduces CCA cell growth. (A) mRNA and protein levels of GCS after suppression by siRNAs for 24 and 48 h. (B) Cell proliferation after GCS suppression by siRNAs for 0–48 h, (C) PPMP (GCS inhibitor), and (D) co-treatment by siRNAs and PPMP. (E,F) The relative mRNA expression of growth-related genes in siGCS-treated KKU-213A or PPMP-treated KKU-213A were determined using qPCR. Values are expressed as the mean ± SEM of three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001 versus siCTRL. The original unedited blot was presented in Figure S1.