Figure 8.

Fibrinogen supplementation stabilizes thrombi formed at hemodilution against fibrino lysis. (A) FITC-fibrinogen (43.5 µg/mL) was added to whole blood (black) or 40% HD blood in the presence of 1 mg/mL FibCLOT^®, RiaSTAP^® or cryoprecipitate. The thrombi were formed by recalcification (10.9 mM CaCl₂) and rotated on at 30 rpm for 90 min. The thrombi were lysed with tPA (1 µg/mL), and lysis was measured as fluorescence release of degradation products. (B). The thrombus lysis rate was calculated by change in fluorescence unit (FU) per minute. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HD. n = 3.