TABLE 1.

DNA probes used in this study

Probe	Characteristics of probes
	Plasmidic origin	Probe size (bp)	Restriction sites or primers	Reference
Replicon typing
RepFIC	pULB2440	967	EcoRI-HindIII	7
RepFIIA	pULB2401	543	PstI	7
RepHI1	pULB2436	2,250	EcoRI-HindIII	7
RepHI2	pULB2433	1,800	EcoRI	7
RepI1	pULB2428	1,100	EcoRI-PstI	7
RepP	pULB2420	750	HaeII	7
RepX	pULB2405	942	HindIII	7
Virulence identification
H6	pIP1350	3,500	HindIII	12
β-Lactamase determination
TEM-1/2a	pBR322	489	5′GAGTACTCACCAGTCACAGAAAAGC3′	35
			5′GACTTCCCGTCGTGTAGATAAC3′
PSE-1	RPL11	586	5′AATGGCAATCAGCGCTTCCC3′	2, 16
			5′GGGGCTTGATGCTCACTCCA3′

^aOligonucleotides and probe TEM-1/2 are homologous to TEM-1 and TEM-2 β-lactamases. IEF allowed us to distinguish between both enzymes.