Fig. 6.

Flow cytometry analysis showed that different leukocyte subsets were elevated in cell numbers in the cerebellum of Ccm3^iECKO mice (CCM3) compared with wild-type controls (WT) during chronic CCM. Activated neutrophils, neutrophil–platelet aggregates and NET levels were elevated in circulation in Ccm3^iECKO mice (CCM3) compared with wild-type controls (WT) during chronic CCM. The following are shown: immune cells (CD45^hi) (A), neutrophils (CD45^hiCD11b⁺Ly6g⁺) (B), inflammatory monocytes (CD45^hiCD11b⁺Ly6g⁺Ly6c^hi) (C), natural killer (NK) cells (CD45^hiCD11b⁻CD19⁻NK1.1⁺CD3⁻) (D), CD4 T cells (CD45^hiCD11b⁻CD19⁻NK1.1⁻γδΔTCR⁻CD3⁺CD4⁺) (E), CD8 T cells (CD45^hiCD11b⁻CD19⁻NK1.1⁻ γδTCR⁻CD3⁺CD4⁻) (F) and B cells (CD45^hiCD11b⁻CD19⁺NK1.1⁻) (G), at P7 (n = 6 per group), P11 (n = 6–8 per group), P13 (n = 5 per group) and P34 (n = 7–8 per group). Low-density granulocytes (LDG) (H), and neutrophil–platelet aggregates (I) as a proportion (%) of circulating neutrophils (CD45⁺CD11b⁺Ly6c^loLy6g⁺) in wild-type controls and Ccm3^iECKO mice (n = 6–7 per group) during chronic CCM at P13. J Neutrophil extracellular traps (NETs) in wild-type controls and Ccm3^iECKO mice (n = 13–15 per group) during chronic CCM, at P15, expressed as arbitrary units (AU). *P < 0.05; **P < 0.01; ***P < 0.001 for comparisons between groups (Mann–Whitney U tests)