TABLE 5.
Phosphorylation of adenosine, NBMPR, hypoxanthine, and NBMP by enzyme extracts from wild-type (RH), adenosine kinase-deficient (Ara-AR), and HGXPRT-deficient (ThxR-1) strains of T. gondii in the presence of ATP or PRPP
| Strain | Cosubstrate | Activity (pmol/min/mg of protein) with the following substratea:
|
|||
|---|---|---|---|---|---|
| Adenosine | NBMPR | Hypoxanthine | NBMP | ||
| RH | ATP | 41.8 ± 1.4 | 4.9 ± 0.5 | NDa | ND |
| PRPP | 0.0 | 0.0 | 10,400 ± 1,230 | 0.0 | |
| Ara-AR | ATP | 0.0 | 0.0 | ND | ND |
| PRPP | 0.0 | 0.0 | 11,970 ± 2,605 | 0.0 | |
| ThxR-1 | ATP | 51.3 ± 5.7 | 3.6 ± 1.3 | ND | ND |
| PRPP | 0.0 | 0.0 | 0.0 | 0.0 | |
a
Values are means ± standard deviations. ND, not determined.