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. 2022 Mar 10;22(6):2506–2513. doi: 10.1021/acs.nanolett.2c00275

Figure 4.

Figure 4

Spatial tolerance of CpG-ODNs binding with flexible DNA linkers. (a) Representation of the CpG-ODNs functionalization to the disk surface in the absence of linkers and in the presence of linkers of 10 or 20 base pairs (bp). (b) Activation assays of RAW 264.7 incubated with Cy5-labeled disk functionalized with two CpG molecules at different distances (7 and 38 nm) and with different linker lengths (0, 10, or 20 bp). Quantitative analysis of the cell-surface expression of the markers CD83 (left) and CD40 (center) in the Cy5 gate assessed by flow cytometry and IL-6 production quantification assessed by ELISA (right) in RAW 264.7. Data normalized compared to cells treated with empty disk. Data are represented as mean of three biological replicates ± standard deviations (n = 3). *p ≤ 0.05; t test 7 nm vs 38 nm CpG disks.