Figure 4.

Effect of JM-00266 on behavior, body temperature and gastrointestinal transit. (A): Cumulative 3-h food intake in 24-h food-deprived mice subjected to an intraperitoneal injection of ACEA (1 mg/kg) alone, in combination with Rimonabant (10 mg·kg⁻¹) or with JM-00266 (20 mg·kg⁻¹). Results are expressed as mean ± SEM (n = 4 per group), * p < 0.05. (B): Effect of AEA (10 mg·kg⁻¹) on rectal temperature in mice treated with JM-00266 or vehicle (20 mg·kg⁻¹). Results are expressed as mean ± SEM (n = 4 per group) * p < 0.05. (C): Effect of ACEA on rectal temperature measured at t = 30 min after injection of ACEA (1 mg·kg⁻¹) in mice treated with JM-00266 (20 mg·kg⁻¹) 20 min earlier. Rimonabant (10 mg·kg⁻¹) or vehicle. Results are expressed as mean ± SEM (n = 4 per group) * p < 0.05. (D): Effect of JM-00266 and Rimonabant on open field exploration. Total ambulatory distance was determined calculating total distance travelled for 10 min. Anxiety was evaluated monitoring total entries in the center of the arena and total time spent in the center on a 10-min period. Results are expressed as mean ± SEM (n = 5 per group). (E): Effect of JM-00266 on gastrointestinal motility. Mice were treated with JM-00266 (10 mg·kg⁻¹) or vehicle 10 min before an injection of anandamide (10 mg·kg⁻¹). Results are expressed as mean ± SEM (n = 3 per group) * p < 0.05.