Figure 4.

Binding of ADPR, cADPR, 8-Br-ADPR, and 8-Br-cADPR to the isolated MHR1/2 domain from drTRPM2. The isolated MHR1/2 domain from drTRPM2 was expressed in E. coli and purified by affinity and size exclusion chromatography and used to determine the binding affinity by isothermal titration calorimetry. The figure shows representative raw data and integrated peaks from 3 experiments per condition (a) ADPR, (b) cADPR, (c) 8-Br-ADPR, and (d) 8-Br-cADPR. The K_D value is expressed as mean ± SEM (n = 3). Raw data from individual experiments can be found in Supplementary Material Figure S2.