Figure 1:

Generation of six1 mutant X. tropicalis line.

A. Schematic outline of genomic six1 locus and Six1 protein. Yellow arrowhead demarcates the relative location of sgRNA target sequence and mutations in this region are expected to disrupt the protein-protein interaction domain (SD).

B. Alignment of wild type and Xtr.six1^em2Horb mutant nucleotide sequence with the −28bp deletion. Underlined region defines where the mutant and wild type sequence are identical after the 28bp deletion. Amino acid sequence is included to show frameshift with resulting stop codon 36 amino acids downstream.