Figure 3:

Changes in gene expression after loss of one (six1^+/−) or two (six1^−/−) alleles of six1.

A. Sections through the otic vesicle (ov) of six1 heterozygous (left) and six1 null larva processed for eya2 expression. Note the smaller domain of eya2 expression in the null ov.

B. Sections through the facial ganglion placode (VIIg) of six1 heterozygous (left) and six1 null larva processed for eya2 expression. Note the discontinuous nature of eya2 expression in the null VIIg.

C. Sections through the branchial arches (ba) of six1 heterozygous (left) and six1 null larva processed for tbx1 expression. Note the lack of tbx1 expression in the null ba.

D. Sections through the ov of six1 heterozygous (left) and six1 null larva processed for tbx1 expression. Note the lack of tbx1 expression in the null ov.

E. Sections through the ov of six1 heterozygous (left) and six1 null larva processed for irx1 expression. Note the lack of irx1 expression in the null neural tube (nt) and ov.

F. Sections through the ov of six1 heterozygous (left) and six1 null larva processed for sobp expression. Note the lack of sobp expression in the null ventral nt, VIIg and ov.

G. Sections through the ov of six1 heterozygous (left) and six1 null larva processed for rnf150 expression. Note the lack of rnf150 expression in the null nt and ov.

H. Sections through the ov of six1 heterozygous (left) and six1 null larva processed for pick1 expression. Note the lack of pick1 expression in the null nt and ov.

All sections are in the transverse plane oriented with dorsal to the top.