Figure 3. Comparison between optimal QLT MS² and Orbitrap MS² methods and their relative quantification precision.

(a) Identification rate of lipids across gradient. (b) Cumulative number of lipid identifications across gradient. (c) Number of lipid identifications within each lipid class from QLT MS² and Orbitrap MS² method. Lipid classes containing > 3 lipids were shown. (d) Histogram of log2 intensity of lipids for QLT MS² and Orbitrap MS² method. The Venn diagram on topright showed the overlap of lipids identified between QLT MS² and Orbitrap MS² method. (e) 2D density plot showing no difference for relative quantification of overlapped lipid identifications between QLT MS² and Orbitrap MS² method. Data were collected in positive mode.